The film revolves around a special team of trained animals. The primary field team consists of three guinea pigs: Darwin, Blaster, and Juarez, as well as a mole, Speckles. The unit’s leader Ben, orders an unauthorized infiltration of the residence of home electronics and appliances magnate, Leonard Saber. Saber’s appliances all have control chips inside them that will activate a function called Sabersense, allowing electronic communication. The team retrieves sensitive information about a scheme called Clusterstorm, which will occur in 48 hours.
However, when Ben’s superior, Kip Killian, arrives for his evaluation, he is angry at Ben for performing an unauthorised mission. The government agent orders the unit shut down. With the help of the humans, Darwin, Juarez, Blaster, Mooch, and Speckles escape, but find themselves in a case bound for a pet shop. Ben and his assistant, Marcie, send a trained cockroach named Harry to infiltrate their hideout, which is now guarded by FBI agents, and recover Darwin’s Personal Digital Assistant, which contained the information acquired from Saber’s computer.
Trapped in the store’s pet rodent display case, G-Force meet Hurley, a guinea pig, Bucky, a hamster, and three mice. Although Blaster and Juarez manage to get themselves sold to a family with plans to return to extract their comrades, Speckles is thrown and seemingly crushed in a garbage truck. Meanwhile, Mooch manages to return to Ben to tell him where his mammalian agents are, but Darwin escapes with Hurley before he can arrive to collect them.